Turn the plate over and repeat this parallel stripe once more. Lift the lid of the plate just enough to insert the loop. Dip the loop into the broth culture containing the mixture of bacteria. Application of streaking. Most bacteria, many other microfungi, and unicellular microalgae, may be most commonly obtained by plating methods such as streak plate method, pour plate method and spread plate method. 2. Uses of the Pour plate method The pour plate technique can determine the number of microbes/mL in a specimen. Friday 2pm: Culture is still turbid white and clumpy. Breif Microbiology Laboratory Theory and application Second Edition, Streak Plate Methods of Isolation (pp. Types of Streak Plate Method Quadrant Streaking: This is the most common method of streaking, where the petri dish is divided into four quadrants and then inoculated. Figure 2. On average, it will give a lower count as heat-sensitive microorganisms may die when they come in contact with a hot molten agar medium. Was the streak plate method effective at diluting the population size in both plates? Using proper technique, flame your loop. Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern. Firstly, users will not be able to grow obligate anaerobes using this method. Streak Plate. What are the advantages of the streak plate method? Sterilize the wire loop. This is to make sure that the individual cells fall apart on the agar medium surface so as separation of the different species takes place. The inoculation loop is first sterilized by passing it through a flame. A bacterial sample is streaked over the surface of a plated agar medium. STREAK PLATE METHOD PRESENTED BY : JYOTSNA VERMA M.Sc (P) BIOCHEMISTRY. The parallel Streak Quadrant Method & Zigzag Streak Quadrant Method are the Quadrant methods whereas the T- Streak method is the three sector method. 1 Two other approaches when making a streak plate are: i to lift the lid vertically (i.e. A commonly used isolation technique is the streak plate. However, the employment of the streaking methods and pattern of streaking varies from lab to lab. THE POUR PLATE AND SPIN PLATE METHODS OF ISOLATION. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. The method uses a single plate of nutrient agar. It is also known as a four-quadrant streak. T-Streaking 3. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.The streaking is done using a sterile tool . Place this order or similar order and get an amazing discount. Saturday 3pm: Streak plate shows streaks of yellow-white colonies (as expected), but also a blue diffuse contaminant which smells very bad. The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. See the instructional photo that appears with this article. Generally, pour plates is the method for counting the number of colony-forming bacteria present in a liquid specimen. Clearly labeling your plates to identify the microorganism only takes a few seconds and will help you avoid a big headache later. Streak as at B to spread the original inoculum over more of the agar. Cool the loop by touching it on the edge of the sterile agar plate. on September 27, 2019 September 27, . Streaking is rapid and ideally a simple process of isolation dilution. The procedure includes a dilution technique which requires spreading a loopful of culture over the agar plate surface. Streak plate and spread plate are two microbial techniques that we use in bacteriology. Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains. Make sure the agar plates are free of droplets of condensed moisture. Dip the loop into the broth culture containing the mixture of bacteria. Questions and Answers on Streak Plate Method Use our database of questions and answers and get quick solutions for your test . Quadrant Streaking It is the most common and preferred method. Module 5 - Abbreviations. Saturday 3pm: Streak plate shows streaks of yellow-white colonies (as expected), but also a blue diffuse contaminant which smells very bad. Another method of separating bacteria is the pour plate method. 2. The streak plate method requires the number of organisms in the inoculums be reduced. Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains. Quadrant Streaking It is the most commonly used and the most preferred method where four equal-sized sections of the agar plate are streaked. Make a dilution series from a sample. Cool the loop by touching it on the edge of the sterile agar plate. Semi-quantitative Streaking 6. What is a pour plate? still directly above the base) the least amount that will allow access of the loop ii to place the lid on the working surface, lift out the base, invert it and inoculate the upwards-facing agar surface. T-Streak The three-phase streaking pattern is known as the T-Streak. Here is the basic technique. Procedure for Streaking a Plate for Isolation: Flame the loop and wire and streak a loopful of broth as at A in the diagram. The approach used depends on personal choice as well as the number of microorganisms found in a sample. In both techniques, we can dilute the sample prior to inoculation. Safety and Lab Guidelines. Quadrant Streaking 2. Reflame the loop and cool it. . Fig.1.1 Different types of streak plates Pour Plate Method: The main principle of this method is the dilution of the inoculum in successive tubes containing liquefied agar medium to permit a thorough distribution of bacterial cells within the medium. The three-sector "T streak" and four-quadrant "streak" techniques are the two most popular streak designs. On the other hand, spread plates allow the isolation of specific clonal colonies. Used to get isolated bacterial colonies on the surface of agar plate. (2012). 5/7/2016 Dr.T.V.Rao MD 28 29. Question: 4. The purpose is to make millions of copies to be able to be seen in a gel. How many types of microbe colonies were present on each of the plates Why might the skin sample agar plate have more types of microbes than the S. cerevisiae plate? Methods of Streak Plate There are many different types of methods used to streak a plate. There are two most commonly used streak patterns, a three sector "T streak " and a four quadrant streak methods. . What is the purpose of the 4 quadrant streak plate method? : Quadrant Streaking, T-Streaking, Continuous Streaking, Radiant Streaking, Semi-quantitative Streaking, Zigzag Streaking. Streak Plate Method Principal: The streak plate method is an isolation procedure used to isolate bacteria from a mixed bacterial culture. This video explains about the different streak plate techniques and the purpose of the technique. Streak the Plates in a defined Manner Remove the loop and close the Petri dish. It is essentially a dilution technique used for isolation of discrete colonies and requires that the number of organisms in the inoculums be reduced. Dip the loop into the broth culture containing the mixture of bacteria. USE Discount . This is similar to what we've seen in our previous attempt at growing this culture Procedure of Spread Plate Technique. Pipette out 0.1 ml from the appropriate desired dilution series onto the center of the surface of an agar plate. Inoculation is the process of inducing immunity against infectious diseases through artificial means. It is a dilution technique in which a bacterial sample is streaked over the agar surface in quadrants so that bacterial cell density gets diluted and individual cells spread over the agar surface. STREAKING: The streak plate method is a rapid qualitative isolation method. Used to dilute bacteria on surface of agar medium until bacteria are spread well enough apart that when they grow, the colonies will will be isolated. Englewood, CO: Morton Publishing. Keep the agar dry. Inoculated a streak on left half of L-agar plate. It is also referred to as the "four-quadrant . Zigzag Streaking Requirements of Streak Plate Method Procedure or Protocol of Streak Plate Method Quadrant Streaking Procedure T-Streaking Procedure Continuous Streaking Procedure BUT T O Words. In the streak plate method of isolation, a bacterial sample (always assumed to be a mixed culture) is streaked over the surface of a plated agar medium. The results should compare favorably with the streak plate and spread plate methods to obtain isolated colonies. Which of the three plates seen in the image would represent a successful quadrant streakmethod? What are the steps of streak plate method? The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. The parallel Streak Quadrant Method & Zigzag Streak Quadrant Method are the Quadrant methods whereas the T- Streak method is the three sector method. How could your methods be improved in the future? Make sure that a small amount of culture is carried over. There are four basic types of streaking methods; Quadrant streaking T-streak Continuous streak Radiant streak Quadrant streaking As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Based on the pattern of streaking, the streak plate method can be classified into 6 types, viz. Both methods require solidified agar plates. The method most often used in the clinical laboratory is the quadrant method. What are the advantages and disadvantages of pour plate method? 23 . 11 terms. The most common way of isolation of pure culture techniques cells on the agar surface to obtain isolated colonies is the streak plate. The pour plate method of counting bacteria is more precise than the streak plate method. 2. 1. As the loop is streaked across the agar surface, more and more bacteria are rubbed off until individual separated . : Quadrant Streaking, T-Streaking, Continuous Streaking, and Radiant Streaking. By using streak plate technique to spread a clinical sample out on the surface of a solid growth medium individual types of bacteria can be isolated. Label first, streak second. The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. There are two most commonly used streak patterns, a three sector "T streak" and four-quadrant streak methods. Three Sector Streak (t streak): Sterilize the wire loop. Do not touch the original inoculum ( ). A Sharpie will also be helpful. 1. O Words) BUEET, 5. Radiant Streaking 5. . Advantages of Pour Plate Technique Inoculated a streak on left half of L-agar plate. Plate C Plate C Collected Lab Data Quadrant Growth on Plate Isolated Colonies Present 1 Heavy No Quadrant Growth on Plate Isolated Colonies Present 2 Heavy No 3 Intermediate Yes, few 4 Scant Yes, many Plate Image End of preview. For this exercise, you will need a spray bottle of 10% Lysol, paper towels, a Nutrient Agar plate, a Bunsen burner, a striker, an inoculation loop, and a stock culture from which to take the inoculum. The streak plate method is a rapid qualitative isolation method. Streak Plate Methods and Protocols. The streak plate method of isolation means to spread the microbes on plated agar media so that the individual cells or colony forming units (CFUs) can become isolated and grow into individual, pure colonies. streak plate method of isolation. Both methods are in use to isolate or separate bacteria from a mixture. Colonies on an agar plate. cells that have been sufficiently isolated grow into. Inoculation - Definition and Methods. Streak Plates vs. Pour Plates: Two methods that can be used in inoculating bacteria into a medium to observe their growth are streak plates and pour plates.The former of these involves gathering bacteria from a suspension into an inoculating loop to be streaked across a portion of the medium, which second, third, and even fourth streaks can be taken from to dilute the bacteria effectively. The Parallel Streak Quadrant method is commonly used in the Labs for the Isolation of Microorganism. Methods of Streak Plate A plate may be streaked using a variety of techniques. 39-44). It provides a simple and rapid method of diluting the sample by mechanical means. Dip the L-shaped glass spreader into alcohol. Finally, make a few streaks in the intact center of the plate. 1. Flame the glass spreader (hockey stick) over a Bunsen burner. Streak as at C. Reflame the loop and cool it. Insert your loop into the tube and remove some inoculum. 43 terms. ContentsStreak Plate Method OverviewObjective of Streak Plate MethodPrinciple of Streak Plate MethodMaterial Required for Streak Plate MethodStreak Plate ProcedureGeneral ProcedureT-Streak or Three Sector Streak MethodFour Quadrant Streak or Quadrant methodQuadrant Streak Method BOther Methods of St.